Asian Journal of Bio Science

A. John De Britto ¹, D. Herin Sheeba Gracelin ¹, T. Leon Stephan Raj ¹, P. Benjamin Jeya Rathna Kumar ¹

Affiliations
1 Department of Plant Biology and Biotechnology, St. Xavier’s College (Autonomous), Palayamkottai (T.N.), IN

Abstract

Solanaceae is one of the families with a number of important agricultural plants as well as many toxic plants. In the present investigation a comparative and comprehensive leaf, branch, fruit and seed have been analyzed and complemented with leaf proteins for identifications. As expected taxonomical characters within Solanum species revealed great morphological differences. Some Solanum species are phenotypically close to each other, but have different taxonomic status. These morphological similarities lead to difficulty in identification of these species. Hence, a study was undertaken to understand the similarities and dissimilarities at morphological and protein level. Molecular weight of the protein of the five species varied from 199.53kD to 1.78kD. The result showed S. surattense and S. trilobatum were closer morphologically whereas, S. surattense and S. melongena were closer at protein level.

Keywords

Solanaceae, SDS-PAGE, Paired Affinity, Similarity, Phylogeny.

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A. John De Britto ¹, G. S. Rekha ¹

Affiliations
1 Plant Molecular Biology Research Unit, PG and Research, Department of Plant Biology and Biotechnology, St.Xavier’s College (Autonomous), Palayamkottai (T.N.), IN

Abstract

In the present study, Acalypha indica L. was collected from ten locations in Tirunelveli hills and the genetic variability was investigated using RAPD-PCR fingerprint and the population which showed high percentage of polymorphism was selected. The selected populations were further subjected to phytochemical analysis. The active principle in these plants was quantified by HPLC analysis. The population which exhibited both high percentage of polymorphism and high amount of active principle was considered as the superior genotype.

Keywords

Acalypha indica, Genetic Variability, RAPD, Active Principle, HPLC, Superior Genotype.

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